Research Assistant III

Department: Location: Health Sciences Center Time Type: Full time Worker Type: Regular Job Req ID: R107493 Minimum Requirements: Associate's Degree in a related field and two (2) years. Grade 3 (Hourly) Position Description: The Research Assistant III in the Brown Cancer Center will be responsible for assisting with studies involving bench work, tissue culture and rodent models to understand the role of an enzyme in cancer development and spread and to examine the effects of inhibition of the enzyme in cancer development. We employ genetic mouse models, primary and transformed cell cultures and molecular biological techniques in our work. Applicants should have previous experience with in vivo experiments with mice and with molecular and cell biology techniques including PCR, Western blotting, tissue culture and cell-based assays. The position will involve providing technical support in a cancer research laboratory using molecular and cell biology techniques and animal models. Duties will include PCR, genotyping, Western blotting, tissue culture, transfection and assistance with mouse colony management and in vivo studies with transgenic and knockout mice. The position will also involve data analysis, laboratory maintenance and training of junior staff. Essential Duties and Responsibilities: Animal studies (including breeding of mice, genotyping, tumor implantation and monitoring and harvesting tissues). Tissue culture (maintenance of cell lines, plating and treating cells with agents in vitro). Cell based assays including measurement of ATP, apoptosis and cell death, flow cytometry, plasmid preparation (for use in silencing proteins), transfection of cells, PCR and Western blotting for protein analysis. Experimental notation, completion of training, training junior staff and supervising students, data preparation and analyses and general laboratory maintenance are also essential duties with this position. Preferred Qualifications: Practical experience with molecular and cellular biology techniques and animal tumor models. Expertise in flow cytometry. Experience with tissue culture including the ability to perform transfections with RNAi and plasmids, propagation of cell lines and clones and testing inhibitors on cells. Experience with Cell based assays will include studies such as ATP measurement, enzyme assays and flow cytometry. Animal studies will include breeding of transgenics, genotyping and xenograft studies in mice. Experience with or expected to learn to present data in the form of graphs and powerpoint slides and assist in the design and modification of experimental techniques. Experience with or expected to perform related library research and prepare materials required for experiments. The Brown Cancer Center encourages candidates to apply who have demonstrated the capacity to create supportive work environments, collaborate effectively on teams, and serve the students we admit and aim to attract. Target Compensation Maximum: $28.55 Target Compensation Minimum: $19.03 Compensation will be commensurate to candidate experience. Equal Employment Opportunity The University of Louisville is an Equal Employment Opportunity employer. The University strives to provide equal employment opportunity on the basis of merit and without unlawful discrimination on the basis of race, sex, age, color, national origin, ethnicity, creed, religion, disability, genetic information, sexual orientation, gender, gender identity or expression, veteran status, marital status, or pregnancy. In accordance with the Rehabilitation Act of 1973 and the Vietnam Era Veteran Readjustment Act of 1974, the University prohibits job discrimination of individuals with disabilities, Vietnam era veterans, qualified special disabled veterans, recently separated veterans, and other protected veterans. The University acknowledges its obligations to ensure affirmative steps are taken to ensure equal employment opportunities for all employees and applicants for employment. It is the policy of the University that no employee or applicant for employment be subject to unlawful discrimination in terms of recruitment, hiring, promotion, contract, contract renewal, tenure, compensation, benefits, and/or working conditions. No employee or applicant for employment is required to endorse or condemn a specific ideology, political viewpoint, or social viewpoint to be eligible for hiring, contract renewal, tenure, or promotion. Assistance and Accommodations Computers are available for application submission at the Human Resources Department located at 215 Central Avenue, Ste 205 - Louisville, Kentucky 40208. If you require assistance or accommodation with our online application process, please contact us by email at employment@louisville.edu or by phone 502-852-6258. The University of Louisville is committed to being a great place to work, learn and invest. It is a place where you will thrive in a community of inclusivity, cultivate your innovative power and work where youll discover your purpose. Join the Cardinal Family today. Learn more about the University at our About Us page.